Assignment: Cell Fractionation Worksheet - Academia Essay Writers

Assignment: Cell Fractionation Worksheet

Assignment: Cell Fractionation Worksheet

Assignment: Cell Fractionation Worksheet


All questions are listed. All data is listed. This was a pre-preformed lab.


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Worksheet for cell Fractionation, Chapter 8


Cellular fractionation is a technique that enables researchers to isolate cellular organelles. Once isolated, the presence and functions of these organelles can be studied biochemically.

  1. Characterization of Mitochondria by Succinic Dehydrogenase Assay

The presence of mitochondria is measured by the ability of the fraction to metabolize succinate and reduce DCPIP (blue) to DCPIPH2 (clear).

Procedure: Described in Powerpoint and in Teams recording


 Sample (Fraction) 1Sample (Fraction) 2
At time t = 0blueblue
After 15 minutes0.8 mm clearing1.2 mm clearing
  1. Which sample (fraction) contains higher concentration of mitochondria? Explain why. (4 points)
  2. Characterization of Mitochondria by Differential Spectral Analysis

Procedure: Described in Powerpoint and in Teams recording


Table 2: absorbance values of scan of fractions 1 and 2.

nmFraction 1Fraction 2
  1. Graph the data above on the same axis with Absorbance on Y-axis ( points) and wavelength on the X-axis. (4 points)
  1. What is the role of CaCl2 used in the spectral analysis? (2 points)

Note: Since the absorbance is directly proportional to the concentration, we will also these abs as concentrations in mM.

  1. Use the graph and determine the concentrations of CytC; CytC reductase, and CytC oxidase and complete table 3 (6 points)

Table 3. Abs values (or mM)

Abs (concentration) mMConcentrations in Fraction 1Concentrations in Fraction2
Cytochrome C (505 nm)  
Cytochrome C reductase (562 nm)  
Cytochrome C oxidase (605 nm)  
  1. Which form of cytochrome C is more predominant in fraction 2? (2 points)
  2. Attended the lab? _____________ (2 points)

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