I would like to either be in a word or pdf file extension I need this done in a few hours so please take this serious.
Thanks
Please make
sure to reference where you obtained these values.
The excerpt on the back is taken from the following journal article on Alzheimer’s
research. For your own information, you may access the tull article through
httQ://pubs.acs.org/journal/mpohbp.
I have only given you a portion of the experimentalsection.
“A Molecular Lirlk between the Active Component of Marijuana and Alzheimer’s Disease
Pathology” Lisa M. Eubanks, Claude J. Rogers, Albert E. Beuscher IV, George F. Koob,
Arthur J. Olsorl, Tobin J. Dickerson, and Kim D. Janda MoA Pnermeceuttcs, 2006, 3 (6),
pp 773-777
.
a) In the experimental section, under the subsection, Acetylcholinesterase
Inhibition Studies, the authors state that “Assays were performed by mixing
AChE, THC, and 340 µM DTNB in 100 mM pH phosphate buffer, pH 8.0,
containing 5o/o methanol. Ignoring the methanol, determine the concentrations of
the conjugate base and the weak acid in the phosphate buffer. The total buffer
concentration is 100 mM. Please indicate the source where you obtained the pKa
value to use in the Henderson-Hasselbalch equation. You must determine the
correct conjugate pair.
b) Calculate how you would prepare 500 ml of this buffer (again, ignore other
components) if you were given the solid chemical form of both the conjugate
base and weak acid. Note: You may purchase various salt forms of the base and
acid. i.e. potassium or sodium. Find molecular weight values for the sodium salt
anhydrous forms.
c) If 50 ml, of 0.1 M HCI were added to 500 ml of this buffer, what would be the
new pH of the solution?
d) Upon reviewing the remainder of the experimental section, list the other buffers
used including their pH and concentration. You may ignore the other component
in the buffer.
e) Do you be Neve phosphate buffer was the best choice to use in this experiment?
Briefly explain your decision.
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